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This technology plays an important role in understanding intracellular metabolism and revealing. The time required to reach isotopic steady state depends on the turnover rate of metabolites in a pathway and the labeling dynamics of upstream metabolites that feed into the pathway. The concept of isotopic steady state is very important because it defines the minimum duration of a 13 c labeling experiment and, thus, of the metabolic steady state.
13 c metabolic flux analysis is a powerful technique for modeling biological systems It takes a certain amount of time before intracellular metabolites reach a constant labeling state, which is referred to as isotopic steady state 46 (fig 13 c metabolic flux analysis is free of all the short comings of the stoichiometric metabolic flux analysis (mfa), though it needs more information in addition to extracellular flux data to have the complete flux distribution
Stoichiometric mfa and flux balance analysis (fba) are restricted in their ability.
13c metabolic flux analysis provides a comprehensive perspective of host metabolism by rigorously quantifying fluxes within intracellular metabolic networks The characterization of production cell lines altered via metabolic engineering or media optimization can help to.
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